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Kerafast/Anti-Actin [mAbGEa] Universal Antibody/EGA004/100ug

Kerafast/Anti-Actin [mAbGEa] Universal Antibody/EGA004/100ug
  • Kerafast/Anti-Actin [mAbGEa] Universal Antibody/EGA004/100ug
商品介绍

ThisuniversalmousemonoclonalantibodywasgeneratedagainstplantactinACT1andrecognizesallplant(arABIdopsisACT1,2,3,4,7,8,11,12),animal(e.g.,humanmuscleandcytoplasmic),fungal,andprotistactins.

ActinexistsasaubiquitousproteininvolvedwithfilamentformationthatmakeuplargeportionsoftheCytoskeleton.Actinfilamentsinteractwithmyosintoassistinmusclecontractionaswellasaidingincellmotilityandcytokinesis.Invertebratestherearethreegroupsofactinisoforms:alpha,betaandgamma.Thealphaactinsarefoundinmuscletissuesandareamajorconstituentofthecontractileapparatus.Thebetaandgammaactinsco-existsinmostcelltypesascomponentsofthecytoskeletonandasmediatorsofinternalcellmotility.

FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.

The InvestigatorPartofTheInvestigator"sAnnexeprogram.

Specifications
ProductType: Antibody
AccessionID: AEC09427
Antigen: ArabidopsisActin(ACT1)
MolecularWeight: 45kDa
Isotype: IgG1
Clonality: Monoclonal
CloneName: mAbGEa(5-16H1C8)
Reactivity: Human,Mouse,Rat,Bovine,Drosophila,Ovine,Plant,Xenopuslaevis,Yeast,Zebrafish
Immunogen: PurifiedArabidopsisactin(ACT1)
SpeciesImmunized: Mouse
PurificationMethod: ProteinGcolumn
Buffer: 0.1MSodiumPhosphate,pH7.4,0.15MNaCl,0.05%(w/v)SodiumAzide
TestedApplications: WB,IF,IHC,ELISA
Storage: -20C
Shipped: Coldpacks
Research
AntibodyReactivity
Actin[mAbGEa]Universal
Actin-1[mAb45a]ACT1,3,4,12
Actin-11[mAb2345a]ACT1,3,4,7,11,12
Actin-2[mAb13a]ACT2,8,11
Actin-8[mAbGPa]ACT1,2,3,4,7,8,11,12
Data

WesternBlotAnalysis

TOP:ReactivitymAbGEawithhuman(HsBandHsA1,native;HsG1andHsA2,recombinant)andplant(At-ACT2,recombinant)actins.ThenumbersontheCoomassieblue–stainedgelindicatetheamountofactinproteinloaded.BOTTOM:ReactivityofmAbGEawithC.intestinaliscytoplasmic-likeactinACTB1(CiB)anddiverseprotistactins.Ac,A.castellanii;Mb,;Cs,C.scutata.About1mgofcruderecombinantactinwasloadedperlane.

Adaptedfrom:Kandasamy,M.K.,etal.(2012)PlantCell24,2041-57.

Provider
FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.
References
  1. Kandasamy,M.K.,Gilliland,L.U.,McKinney,E.C.,andMeagher,R.B.(2001).Oneplantactinisovariant,ACT7,isinducedbyauxinandrequiredfornormalcallusformation.PlantCell13,1541-54.
  2. Kandasamy,M.K.,McKinney,E.C.,andMeagher,R.B.(1999).Thelatepollen-specificactinsinangiosperms.PlantJ18,681-91.
  3. Kandasamy,M.K.,McKinney,E.C.,Roy,E.,andMeagher,R.B.(2012).Plantvegetativeandanimalcytoplasmicactinssharefunctionalcompetenceforspatialdevelopmentwithprotists.PlantCell24,2041-57.

Ifyoupublishresearchwiththisproduct,pleaseletusknowsowecanciteyourpaper.

品牌介绍

Kerafast是位于波士顿的试剂公司,其主要任务是为全球科学界提供特有的实验室研究工具。其产品组合包括细胞系,抗体,小分子,染料等,其中许多在其他地方不可用。自2011年成立以来,全球超过160家机构的研究人员通过Kerafast在线平台提供了创新试剂,在这个平台上,材料可以快速访问,无需通过传统的材料转移协议流程。

在Kerafast,可通过MTA共享的材料,如抗体,细胞系,化合物,病毒载体,质粒等。随着文书工作,延误和成本不断上升,MTA交换过程正在成为学术实验室的沉重负担,因为Kerafast计划可以快速有效地进行补救。

 

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