ThismousemonoclonalantibodywasgeneratedagainstplantactinACT2andrecognizessubclasses1and3ofvegetativeplantactins(arABIdopsisACT2,8,11).
ActinexistsasaubiquitousproteininvolvedwithfilamentformationthatmakeuplargeportionsoftheCytoskeleton.Actinfilamentsinteractwithmyosintoassistinmusclecontractionaswellasaidingincellmotilityandcytokinesis.Invertebratestherearethreegroupsofactinisoforms:alpha,betaandgamma.Thealphaactinsarefoundinmuscletissuesandareamajorconstituentofthecontractileapparatus.Thebetaandgammaactinsco-existsinmostcelltypesascomponentsofthecytoskeletonandasmediatorsofinternalcellmotility.
FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.
PartofTheInvestigator"sAnnexeprogram.
ProductType: | Antibody |
AccessionID: | AEE76148 |
Antigen: | ArabidopsisActin(ACT2) |
MolecularWeight: | 45kDa |
Isotype: | IgG1 |
Clonality: | Monoclonal |
CloneName: | mAb13a |
Reactivity: | Plantactins |
Immunogen: | PurifiedArabidopsisactin(ACT2) |
SpeciesImmunized: | Mouse |
PurificationMethod: | ProteinGcolumn |
Buffer: | 0.1MSodiumPhosphate,pH7.4,0.15MNaCl,0.05%(w/v)SodiumAzide |
TestedApplications: | WB(1-2ug/mL),IF |
Storage: | -20C |
Shipped: | Coldpacks |
Antibody | Reactivity |
Actin[mAbGEa] | Universal |
Actin-1[mAb45a] | ACT1,3,4,12 |
Actin-11[mAb2345a] | ACT1,3,4,7,11,12 |
Actin-2[mAb13a] | ACT2,8,11 |
Actin-8[mAbGPa] | ACT1,2,3,4,7,8,11,12 |
WesternBlotAnalysis
ImmunofluorescenceStainingAnalysis
Adaptedfrom:Kandasamy,M.K.,etal.(2001)PlantCell13,1541-54.
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