ThisrabbitIgGpolyclonalantibodywasgeneratedagainsttheGST-ADAM28-cytoplasmictailandisspecificforthecytoplasmicdomainofmouseADAM28.
TheADAM(ADisintegrinAndMetalloproteaseDomain)familyisauniquegroupofproteinsthathastheABIlitytocleaveorshedextracellularportionsoftransmembraneproteins.Inaddition,ADAMsareinvolvedincell-cellandcell-matrixinteractions(e.g.,fertilization,muscledevelopmentandneurogenesis).
Basedonsequenceidentity,ADAM28ismorecloselyrelatedtosnakevenommetalloproteases(SVMPs)thantopresentlyknownADAMs.NorthernblotanalysisshowsthatADAM28isexpressedintheepididymis,suggestingapossIBLeroleinspermmaturation,andinthelung.MatureADAM28ispresumedtobecatalyticallyactiveandfoundmainlyonthecellsurface.
FromthelaboratoryofCarlP.Blobel,MD,PhD,HospitalforSpecialSurgery.
PartofTheInvestigator"sAnnexeprogram.
| ProductType: | Antibody |
| AccessionID: | AF153350 |
| Antigen: | GST-ADAM28CytoplasmicDomain |
| MolecularWeight: | ~100kDa |
| Isotype: | IgG |
| Clonality: | Polyclonal |
| Reactivity: | Mouse |
| Immunogen: | GST-fusionproteinwiththecytoplasmicdomainofmouseADAM28 |
| SpeciesImmunized: | Rabbit |
| Epitope: | Cytoplasmicdomain |
| PurificationMethod: | Serum,notpurified |
| TestedApplications: | WB(1:500) |
| Storage: | -80C |
| Shipped: | Dryice |
| Antibody | SpeciesReactivity | Specificity |
| ADAM9 | Human | CytoplasmicDomain |
| ADAM9 | Mouse | CytoplasmicDomain |
| ADAM12 | Mouse | CytoplasmicDomain |
| ADAM15 | Human | CytoplasmicDomain |
| ADAM15 | Mouse | CytoplasmicDomain |
| ADAM15 | Human | ExtracellularDomain |
| ADAM15 | Mouse | DisintegrinDomain |
| ADAM17 | Mouse | CytoplasmicDomain |
| ADAM17 | Mouse | Prodomain |
| ADAM19 | Mouse | CytoplasmicDomain |
| ADAM28 | Mouse | CytoplasmicDomain |
| ADAM28 | Mouse | ExtracellularDomain |
| SNX9 | Mouse | CytoplasmicDomain |
WesternBlotAnalysis
WesternblotoflysatesfromCOS-7cellstransfectedwithapcDNAcontrolandwild-typeADAM28(WT).AllsampleswerereducedwithDTTandseparatedona7.5%(w/v)polyacrylamidegel.ProteinsweretransferredtonitrocelluloseandimmunoblottedwithADAM28cytoplasmicdomainantiserum.
Adaptedfrom:HowardL,etal.BiochemJ.2000,348,21-27.
- Howard,L.;MaciewiczR.A.;BlobelC.P.CloningandcharacterizationofADAM28:evidenceforautocatalyticpro-domainremovalandforcellsurfacelocalizationofmatureADAM28.BiochemJ.2000,348,21-27.
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