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当前位置: 首页 > 产品中心 > > Kerafast/Anti-Acetylated Mannan [CCRC-M169, 2C2.D8.E5] Antibody (supernatant)/EGA144/5mL (supernatant)

Kerafast/Anti-Acetylated Mannan [CCRC-M169, 2C2.D8.E5] Antibody (supernatant)/EGA144/5mL (supernatant)

Kerafast/Anti-Acetylated Mannan [CCRC-M169, 2C2.D8.E5] Antibody (supernatant)/EGA144/5mL (supernatant)
  • Kerafast/Anti-Acetylated Mannan [CCRC-M169, 2C2.D8.E5] Antibody (supernatant)/EGA144/5mL (supernatant)
商品介绍

ThismouseIgMkmonoclonalantibodywasgeneratedagainstglycanandrecognizesgumandtomatoacetylatedmannan.

Highlights:

  • Reactswithgumandtomatoacetylatedmannan
  • SuitableforELISA,ImmunolabelingandImmunofluorescenceapplications

Mannanisoneofthemajorconstituentgroupsofhemicelluloseinthewallofhigherplants.ItcompriseslinearorbranchedpolymersderivedfromsugarssuchasD-mannose,D-galactose,andD-glucose.

FromthelaboratoryofMichaelG.Hahn,PhD,UniversityofGeorgia.

Specifications
ProductType: Antibody
Antigen: AcetylatedMannan
AccessionID: CCRCM169
Isotype: IgMk
Clonality: Monoclonal
CloneName: CCRCM169:2C2.D8.E5
Reactivity: Gumandtomato
Immunogen: Pectin/MeBSA
SpeciesImmunized: Mouse
Buffer: Cellculturesupernatant
TestedApplications: ELISA,Immunolabeling,IF
Storage: <1 month="" at="" 4c,="">1monthat-80C
Shipped: Dryice
Documentation

Notes:

Strongreactivitytowardtomatoglucomannan.Weaktomoderatereactivitytoward(1?3)(1?4)-ß-glucan.

ThesemonoclonalantibodiesweredevelopedunderthesponsorshipoftheUSNationalScienceFoundation,throughawardnumberDBI-0421683.Theiruseinbiomasscharacterization,studyofbiomassdeconstructionandquantitationwasdevelopedunderthesponsorshipoftheUSDepartmentofEnergythroughawardsDE-PS02-06ER64304andDE-AC05-00OR22725(BioEenergyScienceCenter).

Provider
FromthelaboratoryofMichaelG.Hahn,PhD,UniversityofGeorgia.
References
  1. RobinE.Young,HeatherE.McFarlane,MichaelG.Hahn,TamaraL.Western,GeorgeW.HaughnandA.LaceySamuels.2008.AnalysisoftheGolgiApparatusinArabidopsisSeedCoatCellsduringPolarizedSecretionofPectin-RichMucilage.ThePlantCellJune2008vol.20no.61623-1638.
  2. DeMartini,JD,Pattathil,S,Avci,U,Szekalski,K,Mazumder,K,Hahn,M.G.,Wyman,CE:Applicationofmonoclonalantibodiestoinvestigateplantcellwalldeconstructionforbiofuelsproduction.EnergyEnviron.Sci.,2011,4,4332-4339.
  3. PattathilS,AvciU,MillerJS,HahnMG.2012.Immunologicalapproachestoplantcellwallandbiomasscharacterization:Glycomeprofiling.In:HimmelM(ed)BiomassConversion:MethodsandProtocols.SpringerScience+BusinessMedia,LLC,NewYork,NY,pp61-72.
  4. A.P.deSouza,D.C.C.Leite,S.Pattahil,M.G.Hahn,M.S.Buckeridge.2013.Compositionandstructureofsugarcanecellwallpolysaccharides:Implicationsforsecondgenerationbioethanolproduction.BioenergyResearch6:564-579.
  5. J.Puhlmann,E.Bucheli,M.J.Swain,N.Dunning,P.Albersheim,A.G.Darvill,andM.G.Hahn.(1994)Generationofmonoclonalantibodiesagainstplantcellwallpolysaccharides.I.Characterizationofamonoclonalantibodytoaterminalalpha-(1,2)-linkedfucosyl-containingepitope.PlantPhysiol.104:699-710.
  6. G.Freshour,R.P.Clay,M.S.Fuller,P.Albersheim,A.G.Darvill,andM.G.Hahn.(1996)Developmentalandtissue-specificstructuralalterationsofthecell-wallpolysaccharidesofArabidopsisthalianaroots.PlantPhysiol.110:1413-1429.
  7. G.Freshour,C.P.Bonin,W.-D.Reiter,P.Albersheim,A.G.Darvill,andM.G.Hahn.(2003)Distributionoffucose-containingxyloglucansincellwallsofthemur1mutantofArabidopsisthaliana.PlantPhysiol.131:1602-1612.
  8. PattathilS,AvciU,BaldwinD,SwennesAG,McGillJA,PopperZ,BoottenT,AlbertA,DavisRH,ChennareddyC,DongR,O"SheaB,RossiR,LeoffC,FreshourG,NarraR,O"NeilM,YorkWS,HahnMG.(2010)AComprehensiveToolkitofPlantCellWallGlycan-DirectedMonoclonalAntibodies.PlantPhysiol.153:514-525.
  9. PattathilS,AvciU,MillerJS,HahnMG.2012.Immunologicalapproachestoplantcellwallandbiomasscharacterization:Glycomeprofiling.In:HimmelM(ed)BiomassConversion:MethodsandProtocols.SpringerScience+BusinessMedia,LLC,NewYork,NY,pp61-72.
  10. PattathilS,AvciU,BaldwinD,etal.2010.Acomprehensivetoolkitofplantcellwallglycan-directedmonoclonalantibodies.PlantPhysiology153,514-525.
  11. PattathilS,HahnMG,DaleBE,ChundawatSP.Insightsintoplantcellwallstructure,architecture,andintegrityusingglycomeprofilingofnativeandAFEXTM-pre-treatedbiomass.JExpBot.2015Jul;66(14):4279-94.

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