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Kerafast/Anti-Actin-7 Antibodies/EGA247/100ug

Kerafast/Anti-Actin-7 Antibodies/EGA247/100ug
  • Kerafast/Anti-Actin-7 Antibodies/EGA247/100ug
商品介绍

ThesemousemonoclonalantibodiesweregeneratedagainstArABIdopsisthalianaActin-7andrecognizeA.thalianaActin-7.

Highlights:

  • ReactwithA.thalianaActin-7(Maydisplayreactivitytowardmultipleotherespecies)
  • SuitableforWesternBlot,ELISAandImmunofluorescenceapplications

Actin-7hasbeenidentifiedinanimals,plantsandprotists.Duringplantgrowthanddevelopment,thephytohormoneauxininducesawidearrayofchangesthatincludecelldivision,cellexpansion,celldifferentiation,andorganinitiation.IthasbeensuggestedthattheactinCytoskeletonplaysanactiveroleintheelaborationoftheseresponsesbydirectingspecificchangesincellmorphologyandcytoarchitecture.ThepromoterandproteinproductofACT7,anAraBDopsisvegetativeactingene,arerapidlyandstronglyinducedinresponsetoexogenousauxin.ACT7isrequiredfortheformationofcallustissue,andisalsoessentialforgerminationandrootgrowthACT7actingeneisalsoexpressedinrapidlydevelopingtissuesandrespondstoexternalstimuli,suchasexposuretohormones.

FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.

Specifications
ProductType: Antibody
Name: Actin-7Antibodies
AccessionID: P53492
Antigen: Actin-7
Isotype: IgG
Clonality: Monoclonal
CloneName: 29G12.G5.G6,33E8.C11.F5.D1,36H8.C12.H10.B6
Reactivity: TestedinA.thaliana.Maydisplayreactivitytowardmultipleotherespecies(ND)
Immunogen: A.thalianaActin-7
SpeciesImmunized: BALB/cmouse
PurificationMethod: ProteinG
Buffer: 0.1MSodiumPhosphate,pH7.4,0.15MNaCl,0.05%(w/v)SodiumAzide
TestedApplications: WB,ELISA,IF(ItisadvisabletouseallthreeMAbsinthefirst-time,qualitativeexperimentalsetuptodeterminewhichisthemostsuitableforaspecificexperiment.ThesemaybeusedyocomplementexcperimentscarriedoutinvolvingmAbGEauniversalanti-actinsantibody.)
Storage: -20C
Shipped: Coldpacks
Provider
FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.
Comments
ItisadvisabletouseallthreeMAbsinthefirst-time,qualitativeexperimentalsetuptodeterminewhichisthemostsuitableforaspecificexperiment.ThesemaybeusedyocomplementexcperimentscarriedoutinvolvingmAbGEauniversalanti-actinsantibody.
References
  1. Kandasamy,M.K.,Gilliland,L.U.,McKinney,E.C.,andMeagher,R.B.(2001).Oneplantactinisovariant,ACT7,isinducedbyauxinandrequiredfornormalcallusformation.PlantCell13,1541-54.
  2. Kandasamy,M.K.,McKinney,E.C.,andMeagher,R.B.(1999).Thelatepollen-specificactinsinangiosperms.PlantJ18,681-91.
  3. Kandasamy,M.K.,McKinney,E.C.,Roy,E.,andMeagher,R.B.(2012).Plantvegetativeandanimalcytoplasmicactinssharefunctionalcompetenceforspatialdevelopmentwithprotists.PlantCell24,2041-57.
  4. L.U.Gilliland,L.C.Pawloski,M.K.Kandasamy,andR.B.Meagher,“ArabidopsisactingeneACT7playsanessentialroleingerminationandrootgrowth,”PlantJournal,vol.33,no.2,pp.319–328,2003.
  5. McDowell,JM,An,Y-q,Huang,S,McKinney,EC,MeagherRB:TheArabidopsisACT7ActinGeneIsExpressedinRapidlyDevelopingTissuesandRespondstoSeveralExternalStimuli.PlantPhysiol.(1996)111:699-711.

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